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live dead tm fixable aqua dead cell stain kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher live dead tm fixable aqua dead cell stain kit
    Live Dead Tm Fixable Aqua Dead Cell Stain Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/live+dead+cell+stain+kit/Bovine+Serum+Albumin/bio_rxiv__64898__2026__06__05__730120-39-4-12
    Average 99 stars, based on 1 article reviews
    live dead tm fixable aqua dead cell stain kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Staining:

    Article Title: Combination of vasculature targeting, hypofractionated radiotherapy, and immune checkpoint inhibitor elicits potent antitumor immune response and blocks tumor progression.
    Article Snippet: Immune phenotyping of mouse samples was performed following staining with: anti- CD45 (eBioscience; clone 30- F11, #48-0451-82), antiCD11b (eBioscience; clone M1/70, #12-0112-82), antiCD11c (eBioscience; clone N418, #69-0114-82), anti- Gr-1 (eBioscience; clone RB6- 8C5, #25-5931-82), anti- MHCII (Biolegend; clone M5/114.15.2, #107606), anti- CD40 (Biolegend; clone 3/23, #124622), anti- MHCI (H- 2Kd) (eBioscience; clone SF1.1.1.1, #17-5957-82), anti- MHCI (H- 2Kb) (Biolegend; clone AF6-88.5, #116518), and antiPD- L1 (Biolegend; clone 10F.962, #124312), anti- CD103 (eBioscience; clone 2E7, #17-1031-82), anti- CD30 (eBioscience; clone 390, #11-0311-82), anti- CD8a (eBioscience; clone 53–6.7, #11-0081-85), anti- CD3 (eBioscience; clone 17A2, #69-0032-82), anti- CD4 (eBioscience, clone GK 1.5, #25-0041-82), anti- PD-1 (eBioscience; clone RMPI30, #46-9981-82), anti- IFNγ (eBioscience; clone XMG1.2, #48-7311-82), and H- 2Ld MuLV gp70 tetramer (MBL, #TS- M521-1). .. For all experiments, a LIVE/DEAD cell stain kit (Invitrogen, #L34966) was used. .. The relative quantification70 of the expression levels of selected genes was carried out by real time- PCR using an ABI PRISM Viia7 (Applied Biosystems).

    Article Title: Agr2-associated ER stress promotes adherent-invasive E. coli dysbiosis and triggers CD103 + dendritic cell IL-23-dependent ileocolitis
    Article Snippet: .. Live/Dead cell stain kit , ThermoFisher , Cat# L34962. .. CD3-eFluor780 (clone ID UCHT1) , ThermoFisher , Cat# 47–0038-42; RRID: AB_1272042.

    Article Title: Distinctive Dynamics and Functions of the CD4+CD25+FOXP3+ Regulatory T Cell Population in Patients with Severe and Mild COVID-19.
    Article Snippet: .. To exclude dead cells, we used a Live/Dead Cell Stain Kit (Invitrogen). .. For intracellular staining, fixation and permeabilization were achieved with the Foxp3/Transcription Factor Staining Buffer Kit (eBioscience) for 15 min, and Abs specific for Foxp3, Ki-67, and CTLA-4 were added for another 15 min (Table I).

    Article Title: Dynamics of NK cell subsets following autologous hematopoietic stem cell transplantation in adult oncologic patients
    Article Snippet: .. After two washes with PBS, PBMCs were initially incubated in 1 mL of a 1/1000 dilution of the LIVE/DEAD Cell Stain Kit (Invitrogen) in PBS, on ice for 30 minutes in the dark. .. Next, cells were washed twice with PBS supplemented with 2.5% bovine serum albumin (BSA) (Sigma-Aldrich) prior to conducting the staining of surface receptors.

    Article Title: The oncogenic kinase TOPK upregulates in psoriatic keratinocytes and contributes to psoriasis progression by regulating neutrophils infiltration.
    Article Snippet: The antibodies using for flow cytometry included BV605-CD45 (Biolegend, cat: 103139), PE-CD3 (Biolegend, cat: 100206), Percp-cy5.5-CD4 (Biolegend, cat: 100434), FITC-γδTCR (Biolegend, cat: 118105), PE-cy7-CD11c (BD Pharmingen, cat: 558079), and APC-Ly6G (Biolegend, cat: 108412). .. Live cells were labeled by using the Live/dead Cell Stain Kit (Thermo Fisher, cat: L34963). .. All statistical analyses were performed by using GraphPad Prism (GraphPad Software, San Diego, USA).

    Article Title: Picolinic acid is a broad-spectrum inhibitor of enveloped virus entry that restricts SARS-CoV-2 and influenza A virus in vivo
    Article Snippet: .. Samples were then centrifuged at 3000 rpm for 5 min at 4°C, the supernatant was discarded and stained with a Live/Dead cell stain kit (Invitrogen, L34966) as per the manufacturer’s instructions. .. Samples were washed once with FACS buffer (PBS with 0.3% BSA) and stained for surface markers CD3 (Invitrogen, 47-0031-82), CD4 (Invitrogen, 11-0042-82), and CD8 (Invitrogen, 78-0081-82) as per manufacturer’s instructions.

    Article Title: Dysfunctional ERG signaling drives pulmonary vascular aging and persistent fibrosis.
    Article Snippet: .. For analysis, the single-cell suspension derived from lungs of WT and ERG CKO mice was obtained as described above and then incubated with antiCD45:PerCp-Cy5.5 (103132, clone 30-F11, Biolegend, San Diego, CA, USA, 1:200 dilution), anti CD11b:BV421 (101235, clone M1/70, Biolegend, San Diego, CA, USA, 1:200 dilution) and anti Ly6G:FITC (127606, Biolegend, San Diego, CA, USA, 1:200 dilution), Ly6G:BV605 (128035, clone 1A8, Biolegend, San Diego, CA, USA, 1:200 dilution), NK1.1:PEcy5 (108715, clone PK136, Biolegend, San Diego, CA, USA, 1:200 dilution), SiglecF:APC-cy7 (565527, cloneE50-2440, BD Biosciences, San Jose, CA, USA, 1:100 dilution), F4/80:APC (123115, clone BM8, Biolegend, San Diego, CA, USA, 1:100 dilution), CD4:APC (100411, clone GK1.5, Biolegend, San Diego, CA, USA, 1:100 dilution), CD19:BUV395 (563557, clone 1D3, BD Biosciences, San Jose, CA, USA, 1:200 dilution), B220:PE (553090, clone RA3-6B2, BD Biosciences, San Jose, CA, USA, 1:200 dilution) and LIVE/DEAD cell stain kit (L23105, Thermo Fisher Scientific, Waltham, MA, USA, 1:1000 dilution). ..

    Article Title: Picolinic acid is a broad-spectrum inhibitor of enveloped virus entry that restricts SARS-CoV-2 and influenza A virus in vivo.
    Article Snippet: .. Samples were then centrifuged at 3000 rpm for 5 min at 4 C, the supernatant was discarded and stained with a Live/Dead cell stain kit (Invitrogen, L34966) as per the manufacturer’s instructions. .. Samples were washed once with FACS buffer (PBS with 0.3% BSA) and stained for surface markers CD3 (Invitrogen, 47-0031-82), CD4 (Invitrogen, 11-0042-82), and CD8 (Invitrogen, 78-0081-82) as per manufacturer’s instructions.

    Incubation:

    Article Title: Dynamics of NK cell subsets following autologous hematopoietic stem cell transplantation in adult oncologic patients
    Article Snippet: .. After two washes with PBS, PBMCs were initially incubated in 1 mL of a 1/1000 dilution of the LIVE/DEAD Cell Stain Kit (Invitrogen) in PBS, on ice for 30 minutes in the dark. .. Next, cells were washed twice with PBS supplemented with 2.5% bovine serum albumin (BSA) (Sigma-Aldrich) prior to conducting the staining of surface receptors.

    Article Title: Dysfunctional ERG signaling drives pulmonary vascular aging and persistent fibrosis.
    Article Snippet: .. For analysis, the single-cell suspension derived from lungs of WT and ERG CKO mice was obtained as described above and then incubated with antiCD45:PerCp-Cy5.5 (103132, clone 30-F11, Biolegend, San Diego, CA, USA, 1:200 dilution), anti CD11b:BV421 (101235, clone M1/70, Biolegend, San Diego, CA, USA, 1:200 dilution) and anti Ly6G:FITC (127606, Biolegend, San Diego, CA, USA, 1:200 dilution), Ly6G:BV605 (128035, clone 1A8, Biolegend, San Diego, CA, USA, 1:200 dilution), NK1.1:PEcy5 (108715, clone PK136, Biolegend, San Diego, CA, USA, 1:200 dilution), SiglecF:APC-cy7 (565527, cloneE50-2440, BD Biosciences, San Jose, CA, USA, 1:100 dilution), F4/80:APC (123115, clone BM8, Biolegend, San Diego, CA, USA, 1:100 dilution), CD4:APC (100411, clone GK1.5, Biolegend, San Diego, CA, USA, 1:100 dilution), CD19:BUV395 (563557, clone 1D3, BD Biosciences, San Jose, CA, USA, 1:200 dilution), B220:PE (553090, clone RA3-6B2, BD Biosciences, San Jose, CA, USA, 1:200 dilution) and LIVE/DEAD cell stain kit (L23105, Thermo Fisher Scientific, Waltham, MA, USA, 1:1000 dilution). ..

    Labeling:

    Article Title: The oncogenic kinase TOPK upregulates in psoriatic keratinocytes and contributes to psoriasis progression by regulating neutrophils infiltration.
    Article Snippet: The antibodies using for flow cytometry included BV605-CD45 (Biolegend, cat: 103139), PE-CD3 (Biolegend, cat: 100206), Percp-cy5.5-CD4 (Biolegend, cat: 100434), FITC-γδTCR (Biolegend, cat: 118105), PE-cy7-CD11c (BD Pharmingen, cat: 558079), and APC-Ly6G (Biolegend, cat: 108412). .. Live cells were labeled by using the Live/dead Cell Stain Kit (Thermo Fisher, cat: L34963). .. All statistical analyses were performed by using GraphPad Prism (GraphPad Software, San Diego, USA).

    Single Cell:

    Article Title: Dysfunctional ERG signaling drives pulmonary vascular aging and persistent fibrosis.
    Article Snippet: .. For analysis, the single-cell suspension derived from lungs of WT and ERG CKO mice was obtained as described above and then incubated with antiCD45:PerCp-Cy5.5 (103132, clone 30-F11, Biolegend, San Diego, CA, USA, 1:200 dilution), anti CD11b:BV421 (101235, clone M1/70, Biolegend, San Diego, CA, USA, 1:200 dilution) and anti Ly6G:FITC (127606, Biolegend, San Diego, CA, USA, 1:200 dilution), Ly6G:BV605 (128035, clone 1A8, Biolegend, San Diego, CA, USA, 1:200 dilution), NK1.1:PEcy5 (108715, clone PK136, Biolegend, San Diego, CA, USA, 1:200 dilution), SiglecF:APC-cy7 (565527, cloneE50-2440, BD Biosciences, San Jose, CA, USA, 1:100 dilution), F4/80:APC (123115, clone BM8, Biolegend, San Diego, CA, USA, 1:100 dilution), CD4:APC (100411, clone GK1.5, Biolegend, San Diego, CA, USA, 1:100 dilution), CD19:BUV395 (563557, clone 1D3, BD Biosciences, San Jose, CA, USA, 1:200 dilution), B220:PE (553090, clone RA3-6B2, BD Biosciences, San Jose, CA, USA, 1:200 dilution) and LIVE/DEAD cell stain kit (L23105, Thermo Fisher Scientific, Waltham, MA, USA, 1:1000 dilution). ..

    Suspension:

    Article Title: Dysfunctional ERG signaling drives pulmonary vascular aging and persistent fibrosis.
    Article Snippet: .. For analysis, the single-cell suspension derived from lungs of WT and ERG CKO mice was obtained as described above and then incubated with antiCD45:PerCp-Cy5.5 (103132, clone 30-F11, Biolegend, San Diego, CA, USA, 1:200 dilution), anti CD11b:BV421 (101235, clone M1/70, Biolegend, San Diego, CA, USA, 1:200 dilution) and anti Ly6G:FITC (127606, Biolegend, San Diego, CA, USA, 1:200 dilution), Ly6G:BV605 (128035, clone 1A8, Biolegend, San Diego, CA, USA, 1:200 dilution), NK1.1:PEcy5 (108715, clone PK136, Biolegend, San Diego, CA, USA, 1:200 dilution), SiglecF:APC-cy7 (565527, cloneE50-2440, BD Biosciences, San Jose, CA, USA, 1:100 dilution), F4/80:APC (123115, clone BM8, Biolegend, San Diego, CA, USA, 1:100 dilution), CD4:APC (100411, clone GK1.5, Biolegend, San Diego, CA, USA, 1:100 dilution), CD19:BUV395 (563557, clone 1D3, BD Biosciences, San Jose, CA, USA, 1:200 dilution), B220:PE (553090, clone RA3-6B2, BD Biosciences, San Jose, CA, USA, 1:200 dilution) and LIVE/DEAD cell stain kit (L23105, Thermo Fisher Scientific, Waltham, MA, USA, 1:1000 dilution). ..

    Derivative Assay:

    Article Title: Dysfunctional ERG signaling drives pulmonary vascular aging and persistent fibrosis.
    Article Snippet: .. For analysis, the single-cell suspension derived from lungs of WT and ERG CKO mice was obtained as described above and then incubated with antiCD45:PerCp-Cy5.5 (103132, clone 30-F11, Biolegend, San Diego, CA, USA, 1:200 dilution), anti CD11b:BV421 (101235, clone M1/70, Biolegend, San Diego, CA, USA, 1:200 dilution) and anti Ly6G:FITC (127606, Biolegend, San Diego, CA, USA, 1:200 dilution), Ly6G:BV605 (128035, clone 1A8, Biolegend, San Diego, CA, USA, 1:200 dilution), NK1.1:PEcy5 (108715, clone PK136, Biolegend, San Diego, CA, USA, 1:200 dilution), SiglecF:APC-cy7 (565527, cloneE50-2440, BD Biosciences, San Jose, CA, USA, 1:100 dilution), F4/80:APC (123115, clone BM8, Biolegend, San Diego, CA, USA, 1:100 dilution), CD4:APC (100411, clone GK1.5, Biolegend, San Diego, CA, USA, 1:100 dilution), CD19:BUV395 (563557, clone 1D3, BD Biosciences, San Jose, CA, USA, 1:200 dilution), B220:PE (553090, clone RA3-6B2, BD Biosciences, San Jose, CA, USA, 1:200 dilution) and LIVE/DEAD cell stain kit (L23105, Thermo Fisher Scientific, Waltham, MA, USA, 1:1000 dilution). ..



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    Image Search Results


    Biocompatibility and cell adhesion of the bone marrow mesenchymal stem cells (BMSCs) in different groups. (A) Cell viability at day 1, 4 and 7 via the CCK-8 assay. (B) Percentage of live cells at day 1 assessed by Live/Dead staining. (C) Proportion of adherent BMSCs. (D) Representative fluorescence images of BMSCs obtained by Live/Dead staining. (E) Representative fluorescence images of adherent BMSCs stained with phalloidin. ∗∗ p < 0.01, and ∗∗∗ p < 0.001, n = 3.

    Journal: Materials Today Bio

    Article Title: 3D-printed titanium scaffolds coated with a multifunctional photothermal-responsive hydrogel promote osteoporotic bone defect repair

    doi: 10.1016/j.mtbio.2026.102879

    Figure Lengend Snippet: Biocompatibility and cell adhesion of the bone marrow mesenchymal stem cells (BMSCs) in different groups. (A) Cell viability at day 1, 4 and 7 via the CCK-8 assay. (B) Percentage of live cells at day 1 assessed by Live/Dead staining. (C) Proportion of adherent BMSCs. (D) Representative fluorescence images of BMSCs obtained by Live/Dead staining. (E) Representative fluorescence images of adherent BMSCs stained with phalloidin. ∗∗ p < 0.01, and ∗∗∗ p < 0.001, n = 3.

    Article Snippet: A live/dead cell double-staining kit (KGA9501-100) was obtained from KeyGENBiotech (Nanjing, China), and a CCK-8 assay kit (TM772) was purchased from Dojindo (Tokyo, Japan).

    Techniques: CCK-8 Assay, Staining, Fluorescence